Ebenezer Chitra
International Medical University, Malaysia
Title: Tumour combating effect of Lactoferricin B peptide
Biography
Biography: Ebenezer Chitra
Abstract
Breast cancer is the most common cancer affecting women; survival depends on early diagnosis and treatment. The common choices of treatment are surgery, radiotherapy, hormonal therapy and chemotherapy. Lactoferricin B (Lfcin B) is a 25-mer natural peptide obtained from milk protein lactoferrin and has demonstrated anti-cancer properties. Tumour was induced in the mammary pad of immunocompromised mice by injecting human breast cancer cells, MDA-MB-231 in the breast pad and allowing to develop into tumors. The establishment of tumor was confirmed by IVIS in vivo imaging system, which detects the GFP fluorescence from the implanted cells. Once the tumors were palpable, they were injected with lactoferricin B peptide intratumorally (3-5 mg/mouse/day) for 3 subsequent days. The mice were sacrificed 15 days post treatment. The harvested tumors injected with peptide showed a remarkable decrease in size and weight compared to tumors injected with vehicle control PBS. Our studies suggest that Lfcin B can be developed as a potential therapy for breast cancer, especially against drug resistant tumour cells.